Mouse Strain information
APB ID 168
Added 15/04/2006
Last Edited 11/05/2014
Strain name: B6.129S4-Elf3tm1Pjh/Apb
Nickname: Elf3 knockout
Strain designation name: B6.129S4-Elf3tm1Pjh/Apb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
Targeted reporter
ES Cell-Line Used TC1 (129/SvJ)
Mode of Inheritance Recessive
1.  Affected Gene Name: E74-like factor 3  
Chromosome 1
Affected Gene Symbol Elf3
Protein expression of altered gene: Nil
Genetic alteration of gene: Exon 2 was disrupted by the insertion of a cassette containing lacZ and neo. An elongated transcript resulting from readthrough of the polyadenylation signal of the selection cassette was detected by Northern blot analysis of mutant mice. While protein analysis was not conducted, the allele was putatively null due to stop codons within the neo gene.
Location on chromosome (bp): 137150158-137155015 bp, - strand
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1101781
Synonyms ESE-1, ESX, jen
Allele Name targeted mutation 1, Paul J Hertzog
Allele Symbol Elf3tm1Pjh
MGI Allele Accession ID MGI:2662485
1.   Transgene: Neomycin resistance gene  
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State embryonic lethality during organogenesis: only about 71% of expected numbers of homozygotes are still alive at E11.5 postnatal lethality: by weaning, only about 27% of expected numbers are still alive. decreased body size: smaller than controls during suckling period although normal in size from E11.5 to birth. After 6 weeks of age, weight gain is normal and mice attain normal size cachexia: 2-3 days before death, mice begin to display a wasted phenotype, appear malnourished, lethargic, watery diarrhoea lethargy: beginning 2-3 days before death abnormal intestinal epithelium morphology: intestinal endothelium fails to transform from pseudostratified to columnar by E17.5. Formation of villi is delayed and those present are misshapen. Connective tissue under the lamina propria of the villi is disorganized. abnormal enterocyte morphology: absorptive enterocytes are morphologically abnormal. Tightly packed with apical cytoplasmic snouts. Microvilli are short. Increased numbers of mitochondria and ribosomes. abnormal intestinal goblet cells: reduced numbers of goblet cells producing sialomucin. diarrhoea: watery diarrhea in mice that die starting 2-3 days before death.
Phenotype Heterozygous State No over phenotype
Original Genetic Background 129S4/SvJ x C57BL/6
Genetic Background Currently Maintained C57BL/6
Coat Colour Black
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain Cancer research - Elf3 KO has gut differentiation defect Inflammation - Elf3 is induced in a variety of cells with inflammatory stimuli
Applicable Research Areas Cell biology
Developmental biology
Cancer
Immunology and inflammation
Keywords
  • ETS transcription factor
  • intestine
  • TGF
  • epithelium
  • differentiation
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 6 mice)

APB stock genotyped verified by: The depositor
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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