Mouse Strain information
APB ID 161
Added 09/03/2006
Last Edited 11/05/2014
Strain name: B6.129P2-Dll3tm1Rbe/Apb
Nickname: D3∆
Strain designation name: B6.129P2-Dll3tm1Rbe/Apb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
ES Cell-Line Used CGR8
Mode of Inheritance Recessive
1.  Affected Gene Name: delta-like 3  
Chromosome 7
Affected Gene Symbol Dll3
Protein expression of altered gene: Nil
Genetic alteration of gene: A PGKneo cassette was inserted into the Dll3 locus replaceing exons 5-8
Location on chromosome (bp): 29078572-29087257 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1096877
Allele Name Dll3 targetted mutation 1, Rosa Beddington
Allele Symbol Dll3tm1Rbe
MGI Allele Accession ID MGI:2178443
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygous mutants have shortened tail and trunk, with disorganised vertebrae and costal defects resulting from defective segmentation in the embryo and delayed and irregular somite formation.
Phenotype Heterozygous State None
Original Genetic Background CGR8 ES cells (120Ola) injected into C57BL/6 blastocysts
Genetic Background Currently Maintained C57BL/6
Coat Colour Black
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols To Distinguishthe DII3 wildtype allele from DII3 tm1Rbe
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Yes
Research value of this Strain Models human genetic disease spondylocostal dystosis MIM 277300
Applicable Research Areas Models for human disease
Developmental biology
Keywords
  • Dll3
  • somitogenesis
  • spondylocostal dysostosis
  • abnormal vertbral segmentation
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 14 mice)

APB stock genotyped verified by: The depositor
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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