Home - Australian Phenomics Facility

Mouse Strain information

APB ID

160

Added

08/03/2006

Last Edited

11/05/2014

Strain name:

B6.129P2-Cited1tm1Dunw/Apb

Strain designation name:

B6.129P2-Cited1tm1Dunw/Apb

Genetics

Strain Types

Mutant strain
Congenic

Genetic Details

Targeted knockout

ES Cell-Line Used

E14.2

Mode of Inheritance

X-linked

1. Affected Gene Name: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domian 1

Chromosome	X
Affected Gene Symbol	Cited1
Protein expression of altered gene:	Nil
Genetic alteration of gene:	A loxP site was inserted 51bp upstream from the ATG and Cre/lox recombination used to replace the entire coding region with a GFP-PGKneo cassette.
Location on chromosome (bp):	96848743-96853525 bp, - strand (From Ensembl annotation of NCBI Build 34)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:108023
Synonyms	Msg1, ER154
Allele Name	Cited1 targeted mutation 1, Sally L Dunwoodie
Allele Symbol	Cited1tm1Dunw
MGI Allele Accession ID	MGI:3028946

Mutant Construction Technique

CRE recombinase-loxP technology

Phenotype

Phenotype Homozygous State

Results in impaired embryonic growth and survival due to defects in trophoblast and placental development.

Phenotype Heterozygous State

Male hemizygotes have the same phenotype as the female homozygotes. Female heterzygotes have a variable phenotype depending on which parent the Cited1tm1Dunw allele is inherited from. Heterozygous females with paternally inherited Cited1tm1Dunw have no placental phenotype, whereas heterozygous females with maternally inherited Cited1tm1Dunw have the same placental phenotype as homozygotes

Original Genetic Background

129P2/OlaHsd ES cells injected in C57BL/6 blastocysts

Genetic Background Currently Maintained

C57BL/6

Coat Colour

Black

Eye Colour

Black

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

Cited1 triplex to distinguish the Cited1 wildtype allele from Cited1 tm1Dunw

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	No	No
Are homozygous mice fertile?	No	No
*Are heterozygotes / hemizygotes fertile?**	Yes	No
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Generations Backcrossed	7
Sib-Mated Generations	9
Mating Scheme	Cited1tm1Dunw/Y x +/Cited1tm1Dunw
Homozygous Matings Required?	No
Development Summary	Chimeras made between ES cells (129P2/OlaHsd) and C57BL/6. Chimeras mated with C57BL/6. Seven backcrosses to C57BL/6. Cited1tmDunw/Y x +/Cited1tm1Dunw
Age of Reproductive Maturity	8 weeks
Age of Reproductive Decline	12 months
Male Fertility Compromised?	No
Average Number of Pups at Birth	5.0
Average Number of Pups Surviving to Weaning	4.0
Weaning Age	4 weeks
Number of Litters in Lifetime	6
Breeding Performance	Good
Number of Litters in Lifetime	6
Special Husbandry Requirements	None

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Yes

Research value of this Strain

Models human placental insufficiency

Applicable Research Areas

Developmental biology

Keywords

Cited1
placenta
growth restriction
embryonic
placenta

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 13 mice)

APB stock genotyped verified by:

The depositor

Are live mice available?

Unknown

MTA needs to be signed?

Yes