Mouse Strain information
APB ID 160
Added 08/03/2006
Last Edited 11/05/2014
Strain name: B6.129P2-Cited1tm1Dunw/Apb
Strain designation name: B6.129P2-Cited1tm1Dunw/Apb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
ES Cell-Line Used E14.2
Mode of Inheritance X-linked
1.  Affected Gene Name: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domian 1  
Chromosome X
Affected Gene Symbol Cited1
Protein expression of altered gene: Nil
Genetic alteration of gene: A loxP site was inserted 51bp upstream from the ATG and Cre/lox recombination used to replace the entire coding region with a GFP-PGKneo cassette.
Location on chromosome (bp): 96848743-96853525 bp, - strand (From Ensembl annotation of NCBI Build 34)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:108023
Synonyms Msg1, ER154
Allele Name Cited1 targeted mutation 1, Sally L Dunwoodie
Allele Symbol Cited1tm1Dunw
MGI Allele Accession ID MGI:3028946
Mutant Construction Technique CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State Results in impaired embryonic growth and survival due to defects in trophoblast and placental development.
Phenotype Heterozygous State Male hemizygotes have the same phenotype as the female homozygotes. Female heterzygotes have a variable phenotype depending on which parent the Cited1tm1Dunw allele is inherited from. Heterozygous females with paternally inherited Cited1tm1Dunw have no placental phenotype, whereas heterozygous females with maternally inherited Cited1tm1Dunw have the same placental phenotype as homozygotes
Original Genetic Background 129P2/OlaHsd ES cells injected in C57BL/6 blastocysts
Genetic Background Currently Maintained C57BL/6
Coat Colour Black
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols Cited1 triplex to distinguish the Cited1 wildtype allele from Cited1 tm1Dunw
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Yes
Research value of this Strain Models human placental insufficiency
Applicable Research Areas Developmental biology
Keywords
  • Cited1
  • placenta
  • growth restriction
  • embryonic
  • placenta
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 13 mice)

APB stock genotyped verified by: The depositor
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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