Mouse Strain information
APB ID 159
Added 07/03/2006
Last Edited 11/05/2014
Strain name: STOCK H2k2 Zap70mur/AnuApb
Nickname: Murdoch
Strain designation name: STOCK H2k2 Zap70mur/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: Zap70, zeta-chain (TCR) associated protein kinase  
Chromosome 1
Affected Gene Symbol Zap70
Protein expression of altered gene: Nil
Genetic alteration of gene: ENU induced mutation. A⇒T at nucleotide 1190; I366F
Location on chromosome (bp): 36706371-36727362 bp, + strand (From Ensembl annotation of NCBI Build 36)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:99613
Synonyms Zap-70, Srk, TZK
Allele Name Murdoch
Allele Symbol Zap70mur
MGI Allele Accession ID MGI:3614796
Ensembl Gene ID: ENSMUSG00000026117
Transcript ID: ENSMUST00000027291
Transcript name: Zap70-201
bp change: A to T at position 1190 on cDNA
Exon number: 9
Exon ID: ENSMUSE00000448670
cDNA position: 1190
Amino acid change: Isoleucine to Phenylalanine at position 366
Sequence:
1160CGCATGCGCAAGAAGCAGATTGACGTGGCCATCAAGGTGCTGAAGCAGGGCACAGAGAAGGcDNA (forward strand)
357-R--M--R--K--K--Q--I--D--V--A--I--K--V--L--K--Q--G--T--E--K--protein
Version: NCBI m37, Ensembl build 55
1.  Affected Congenic Gene Name: histocompatibility-2, MHC  
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Reduced naïve CD4⁺ and CD8⁺ T cells which are hyperactivated, reduced CD4⁺ cells, defect in TCR signal transduction, impaired but not severe response to anti-TCR stimulation, T cell intrinsic defect, Elevated serum levels of IgG2b
Original Genetic Background C57BL/6
Genetic Background Currently Maintained B10.BR x C57BL/6
Strain identification
How is this strain characterised? Genotyping
PCR protocols Murdoch (Mrd)
Murdoch (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? Yes
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • T cell
  • zap70
  • B cell
  • TCR
  • signalling
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 12 mice)

APB stock genotyped verified by: The APF
Are live mice available? No
MTA needs to be signed? Yes


   

 


Build: 2017.09.11 Non-profit supported by the Australian government  |  © Copyright 2017 Australian Phenomics Facility Last updated: 22/09/2017