Mouse Strain information
APB ID 1514
Added 04/07/2007
Last Edited 11/05/2014
Strain name: B6.129S4-Nos3tm1Plh/AnuApb
Nickname: eNOS -/-
Strain designation name: B6.129S4-Nos3tm1Plh/AnuApb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
ES Cell-Line Used J1
Mode of Inheritance Recessive
1.  Affected Gene Name: nitric oxide synthase 3, endothelial cell  
Chromosome 5
Affected Gene Symbol Nos3
Protein expression of altered gene: Nil
Genetic alteration of gene: A neomycin cassette replaced 3 exons that encode the NADPH ribose and adenine binding sites of the protein (amino acids 1010 - 1144). Western blots of protein extracts of brain, heart, lung, and aorta/vena cava from homozygous mutant mice showed no detectable encoded protein.
Location on chromosome (bp): 23870628-23890292 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97362
Synonyms 2310065A03Rik, ecNOS, eNOS, Nos-3
Allele Name targeted mutation 1, Paul L Huang
Allele Symbol Nos3tm1Plh
MGI Allele Accession ID MGI:2150145
Allele synonyms: eNOS KO, eNOS-, NOSIII-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygotes for targeted null mutations exhibit reduced survival, hypertension, inhibited basal vasodilation, insulin resistance, fewer mitochondria, reduced heart rate, impaired ovulation and, in some, shortened limbs.
Original Genetic Background 129S4/SvJae
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Developmental biology
Cardiovascular
Endocrine deficiency
Reproduction
Keywords
  • oocytes
  • limb
  • gonadotrophin
  • hypertension
Additional Information Female reproduction / fertility is affected
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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