Mouse Strain information
APB ID 1509
Added 04/07/2007
Last Edited 11/05/2014
Strain name: B6;129-Cfbtm1Hrc/Apb
Nickname: H2-Bftm1Hrc
Strain designation name: B6;129-Cfbtm1Hrc/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: complement factor B  
Chromosome 17
Affected Gene Symbol Cfb
Protein expression of altered gene: Nil
Genetic alteration of gene: A neomycin resistance cassette replaced exons 3 through 7 of the gene. Northern blots of liver from homozygous mutant mice showed no detectable full-length transcript, but did show a smaller, truncated transcript when probes encompassing segments 5' or 3' to the deleted segment were used. Small amounts of truncated, but not intact, protein were detected in peritoneal macrophages from homozygous mutant mice. However, sera from homozygous mutant mice showed no detectable protein activity.
Location on chromosome (bp): 34993319-34999463 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:105975
Synonyms alternative-complement pathway C3/C5 convertase, B, Bf, Bf, Factor B, FB, H2-Bf, properdin
Allele Name targeted mutation 1, Harvey R Colten
Allele Symbol Cfbtm1Hrc
MGI Allele Accession ID MGI:1861129
Allele synonyms: Bf-, Factor B-, fB-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygotes for targeted null mutations lack the alternative complement pathway, and have reduced overall complement activity. Abnormal complement cascade: total hemolytic complement activation was reduced 50% in mutant sera. Impaired complement alternative pathway: * serum from mutant mice showed no evidence of C3 deposition on zymosan. * serum from mutant mice did not support in vitro cleavage of C3 by cobra venom factor. * alternative pathway-dependent hemolytic activity was undetectable in mutant serum.
Genetic Background Currently Maintained C57BL/6 x 129
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • complement
  • C3
  • alternative pathway
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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