Mouse Strain information
APB ID 1508
Added 04/07/2007
Last Edited 11/05/2014
Strain name: B6.Cg-Gzmatm1Simn Gzmbtm1Ley Fastm1Osa/Apb
Nickname: FAS.AB
Strain designation name: B6.Cg-Gzmatm1Simn Gzmbtm1Ley Fastm1Osa/Apb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
Mode of Inheritance Dominant
1.  Affected Gene Name: Fas (TNF receptor superfamily member)  
Chromosome 19
Affected Gene Symbol Fas
Protein expression of altered gene: Nil
Genetic alteration of gene: A BetaGal-neo cassette was used to delete a portion of exon 9, which codes for the cytoplasmic domain essential for Fas-mediated apoptosis. Northern blot analysis revealed the expression of a truncated mRNA, representing transcripts from exons 1 though 8 in the thymus, liver, and heart. Immunoprecipitation analysis showed an absence of the wild-type protein as well as a faint presence of what the authors speculated to be a non-functional Tnfrsf6-BetaGal fusion protein.
Location on chromosome (bp): 34365199-34402260 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

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MGI:95484
Synonyms APO-1, CD95, TNFR6, Tnfrsf6
Allele Name targeted mutation 1, Osaka University Medical School
Allele Symbol Fastm1Osa
MGI Allele Accession ID MGI:1861923
Allele synonyms: Fas-, Tnfrsf6tm1Osa
2.  Affected Gene Name: granzyme A  
Chromosome 13
Affected Gene Symbol Gzma
Protein expression of altered gene: Nil
Genetic alteration of gene: The gene was disrupted by insertion of a neomycin resistance cassette into exon 4 by homologous recombination. Western blot analysis confirmed the absence of protein product and cytochemical analysis demonstrated the absence of enzyme activity in cytotoxic T cells in homozygous mutant animals.
Location on chromosome (bp): 113884035-113891189 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:109266
Synonyms BLT esterase, Ctla-3, Ctla3, H factor, Hanukah factor, Hf, SE1, serine esterase 1, TSP-1, TSP1
Allele Name targeted mutation 1, Markus M Simon
Allele Symbol Gzmatm1Simn
MGI Allele Accession ID MGI:2449926
Allele synonyms: GrzA-, gzm A-, gzmA-
3.  Affected Gene Name: granzyme B  
Chromosome 14
Affected Gene Symbol Gzmb
Protein expression of altered gene: Unknown
Genetic alteration of gene: A PGK-neomycin resistance cassette replaced a 350bp AvrII fragment starting within the 5' untranslated region (22 bp upstream from the initiation codon), extending through exon 1 and part of intron 1.
Location on chromosome (bp): 56877693-56881097 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:109267
Synonyms CCP-1/C11, CCP1, Ctla-1, Ctla1
Allele Name targeted mutation 1, Timothy J Ley
Allele Symbol Gzmbtm1Ley
MGI Allele Accession ID MGI:1857156
Allele synonyms: Granzyme B-, GrB KO, GrzB-, gzmB-, Gzmb-/+PGK-neo
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Increased susceptibility to parasitic infection: decreased clearance and survival after infection with Trypanosoma cruzi.
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
PCR protocols FasAB_GranzymeB(ASD)
FasAB_GranzymeA(ASD)
Fas(-/-)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Virology
Cell biology
Apoptosis
Immunology and inflammation
Keywords
  • infection
  • T cell
  • Natural Killer Cell
  • cytolysis
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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