Mouse Strain information
APB ID 1504
Added 04/07/2007
Last Edited 11/05/2014
Strain name: B6.129P2-Il18tm1Aki/AnuApb
Nickname: B6.IL-18-/-
Strain designation name: B6.129P2-Il18tm1Aki/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: interleukin 18  
Chromosome 9
Affected Gene Symbol Il18
Protein expression of altered gene: Nil
Genetic alteration of gene: eplacement of exons 3, 4 and 5 with a neomycin resistance gene inserted in frame into exon 3. By Northern blot analysis, mRNA could not be detected in macrophages derived from homozygous mutant mice. By ELISA assay, protein cound not be detected in serum of homozygous mutant mice after intravenous injection of lipopolysaccharide.
Location on chromosome (bp): 50383378-50389942 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:107936
Synonyms Igif, Il-18
Allele Name targeted mutation 1, Shizuo Akira
Allele Symbol Il18tm1Aki
MGI Allele Accession ID MGI:2136769
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State In Propionibacterium acnes (P. acnes)-primed IL-18-deficient mice, LPS-induced IFNgamma production was markedly reduced, despite normal IL-12 induction. Natural killer cell activity was significantly impaired. Th1 cell response after injection of P. acnes or Mycobacterium bovis (bacillus Calmette-Guerin [BCG]) was significantly reduced.
Original Genetic Background C57BL/6 x 129P2
Genetic Background Currently Maintained C57BL/6 x 129P2
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Cell biology
Immunology and inflammation
Keywords
  • T cell
  • interferon
  • Natural Killer
  • IL-12, IL12
Additional Information Written permission required from Dr Akira for use of mice.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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