Mouse Strain information
APB ID 1494
Added 03/07/2007
Last Edited 11/05/2014
Strain name: B6;129S-C1qatm1Mjw/Apb
Nickname: B6.C1q-/-
Strain designation name: B6;129S-C1qatm1Mjw/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: complement component 1, q subcomponent, alpha polypeptide  
Chromosome 4
Affected Gene Symbol C1qa
Protein expression of altered gene: Nil
Genetic alteration of gene: A neomycin selection cassette was inserted into exon 1 of the gene. Northern blot analysis on samples derived from spleen and liver of homozygous mice demonstrated that no transcript is produced from this allele. Western blot and ELISA analysis confirmed that no protein was detected in samples derived from homozygous mice.
Location on chromosome (bp): 136451832-136454718 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:88223
Synonyms C1q
Allele Name complement component 1, q subcomponent, alpha polypeptide; targeted mutation 1, Mark J Walport
Allele Symbol C1qatm1Mjw
MGI Allele Accession ID MGI:2158701
Allele synonyms: C1q-, C1qa-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Premature death: 15% die at 4 - 6 months of age Increased anti-nuclear antigen antibody level: * mutants develop high titers of anti-nuclear antibodies Increased anti-histone antibody level. Glomerulonephritis: * Background Sensitivity: severe proliferative crescentic glomerulonephritis evident in 27% mice. * Background Sensitivity: striking feature is presence of multiple apoptotic bodies in diseased glomeruli. * among mutants without glomerulonephritis, significantly greater numbers of glomerular apoptotic bodies are seen than in controls. Increased susceptibility to bacterial infection: * animals challenged with virulent pneumococci develop bacteremia and pneumonia after passive immunization with human IgG1 or human IgG2 unlike wild-type mice that are protected after immunization.
Original Genetic Background 129S/SvEv
Genetic Background Currently Maintained C57BL/6 x 129
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • glomerulonephritis
  • autoimmunity
  • anti-nuclear antibodies
  • infection
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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