Mouse Strain information
APB ID 123
Added 06/02/2006
Last Edited 11/05/2014
Strain name: B10JAnu.Cg-H2k2 Cd40lgtm1Flv Tg(TcrHEL3A9)1Mmd/AnuApb
Nickname: CD40L -/- TCR
Strain designation name: B10JAnu.Cg-H2k2 Cd40lgtm1Flv Tg(TcrHEL3A9)1Mmd/AnuApb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
Transgenic
Mode of Inheritance Recessive
1.  Affected Gene Name: CD40 ligand  
Chromosome X
Affected Gene Symbol Cd40lg
Protein expression of altered gene: Nil
Genetic alteration of gene: A 500 base pair BamHI-XbaI fragment, corresponding to bases 368 through 417 of the cDNA, was deleted by the insertion of a neomycin selection cassette. The encoded protein was undetected via flow cytometry on the surface of activated T cells derived from homozygous mutant mice.
Location on chromosome (bp): 54465320-54477219 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:88337
Synonyms CD154, Cd40L, gp39, HIGM1, IMD3, Ly-62, T-BAM, Tnfsf5
Allele Name targeted mutation 1, Richard A Flavell
Allele Symbol Cd40lgtm1Flv
MGI Allele Accession ID MGI:2182733
Allele synonyms: CD154-, CD154KO, CD40L def., CD40L-, Cd40lg-
1.   Transgene: transgene insertion 1, Mark M Davis  
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygous mice display: - immune system, lymphoid development/B lymphocyte defects: - B cells failed to undergo isotype switching - failed to produce germinal centers in spleen in response to T-dependent antigens - reduced serum immunoglobulin concentrations (IgG1, IgG2a, IgG2b, IgG3), at 12 weeks of age, but not at 6 weeks of age - decreased IgM response to T-dependent antigens - failed to produce antigen-specific IgG1 after immunization - immune system, no defect detected: - no alterations in B or T lymphocyte development - T lymphocytes responded normally to mitogen stimulation - B lymphocytes had normal responses to T-independent antigens
Original Genetic Background 129S2
Genetic Background Currently Maintained C57BL/10J
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Applicable Research Areas Cell biology
Immunology and inflammation
Keywords
  • B cell
  • T cell
  • immunoglobulin
  • germinal centre
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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