Mouse Strain information
APB ID 120
Added 06/02/2006
Last Edited 11/05/2014
Strain name: B6.129P2-Cblbtm1Pngr/AnuApb
Nickname: Cblb KO
Strain designation name: B6.129P2-Cblbtm1Pngr/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: Casitas B-lineage lymphoma b  
Chromosome 16
Affected Gene Symbol Cblb
Protein expression of altered gene: Nil
Genetic alteration of gene: A neomycin cassette replaced the region encoding a portion of the tyrosine kinase-binding and RING finger domains, corresponding to amino acids 300 through 340 of the encoded protein. Western blot analysis showed an absence of protein expression in lymph nodes and splenocytes from homozygous mice.
Location on chromosome (bp): 52031662-52208160 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:2146430
Synonyms Cbl-b
Allele Name targeted mutation 1, Josef M Penninger
Allele Symbol Cblbtm1Pngr
MGI Allele Accession ID MGI:2180570
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygotes for targeted null mutations exhibit elevated IL2 production by T cells, develop spontaneous autoimmunity, and are highly susceptible to experimental autoimmune encephalomyelitis.
Original Genetic Background 129P2/OlaHsd
Genetic Background Currently Maintained B6/129 intercross, bred with wild type B10.BR
Strain identification
How is this strain characterised? Genotyping
PCR protocols Cbl-b KO
Cbl-b WT
Cbl-b 2007/HOT START MIX
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Applicable Research Areas Cell biology
Developmental biology
Immunology and inflammation
Keywords
  • IL-2
  • T cell
  • encephalomyelitis
  • autoimmune
  • signal transduction
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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