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Mouse Strain information
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| APB ID | 113 | |||
| Added | 06/02/2006 | |||
| Last Edited | 11/05/2014 | |||
| Strain name: | B6.Cg-Ptprca Tg(BCL2)22Wehi/WehiAnuApb | |||
| Nickname: | Bcl-2 :B6:Ly5a | |||
| Strain designation name: | B6.Cg-Ptprca Tg(BCL2)22Wehi/WehiAnuApb | |||
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Genetics
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| Strain Types |
Mutant strain
Congenic |
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| Genetic Details |
Congenic
Transgenic |
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| Mode of Inheritance | Recessive | |||
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| Mutant Construction Technique | None | |||
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Phenotype
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| Phenotype Homozygous State | During an immune response, hypermutation of immunoglobulin genes in B cells proliferating within germinal centres (GCs) generates variant antibodies that react with higher affinity against either foreign or self antigens. Several experiments suggest that self-reactive B cells may be censored at this stage of the immune response, but the rarity of these cells and the dynamic nature of GC reactions have prevented direct analysis. We have developed a new approach to visualize the fate of antigen-specific B cells during GC reactions by seeding an ongoing immune response with lysozyme-specific B cells from immunoglobulin-gene transgenic animals. Administration of soluble antigen at the peak of the GC response rapidly eliminates lysozyme-specific GC B cells in two waves of apoptosis, one within the GC and a second in cells that have redistributed to lymphoid zones that are rich in T cells. Elimination of these cells is inhibited by constitutive expression of the follicular lymphoma proto-oncogene bcl-2. These findings reveal censoring steps that may normally prevent affinity maturation of autoantibodies to systemic autoantigens, and might be used by pathogenic microorganisms or in clinical strategies to interfere with antibody responses. | |||
| Original Genetic Background | C57BL/6 x SLJ | |||
| Genetic Background Currently Maintained | C57BL/6 | |||
| Coat Colour | Black | |||
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Strain identification
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| How is this strain characterised? |
Genotyping
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| PCR protocols |
Ly5a/b (amplifluor)
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Fertility and Strain maintenance
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Relevant bibliographic / database references
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General information
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| Associated IP rights? | No | |||
| Does it Model a Human Condition? | No | |||
| Applicable Research Areas |
Research tools
Immunology and inflammation Developmental biology Cell biology Apoptosis Neurobiology |
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| Keywords |
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| Is the strain available in any form to other researchers | Yes | |||
| APB stock stored as: |
Cryopreserved sperm (from 10 mice)
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| APB stock genotyped verified by: | Unverified | |||
| Are live mice available? | No | |||
| MTA needs to be signed? | Yes | |||
