Embryo Cryopreservation

Print version
Embryo Cryopreservation

Embryo Cryopreservation

Acceptance to cryopreserve and store strains is determined by the Scientific Advisory and Access Committee of the APN. Unique strains and strains with high value to the community will be given preference.

 

Cryopreservation is available at a reduced rate:

    • for strains that cannot be cryopreserved in the sperm format.

    and

    • the strains are distributable to other researchers within Australia.

 

Please contact the APB Curator if you would like your strain(s) considered for embryo cryopreservation.

A decision will be made based on the information provided.

The decision will be referred to the Scientific Advisory Committee (SAC) of the APN. The SAC decision will be final.

 

The ongoing maintenance costs, e.g. liquid nitrogen, are also supported.

However, the depositor will need to pay for shipping of the mice to the ARC and the caging of breeders to allow the production of 400-600 embryos.

 

For those strains that are not freely distributable depositors will be charged the full price for the cryopreservation, on going maintenance (a yearly fee) and for shipment.

 

The full cryopreservation cost will not be charged for strains on delayed release however if the block on distribution is not removed at the completion of the agreed period of time, the depositor will be charged for cryopreservation and ongoing maintenance costs.

 

Requests for cryopreservation will be processed in the order the requests are received.

Photo provided by Dr L. Gabriel Sanchez-Partida,

Monash University

Mouse Requirements

A minimum of 4 fertile male mice and 8 fertile female mice, 8-12 weeks of age are required. Mice will be shipped to ARC at the researcher or animal facilities expense. Shipping details will be provided once the strain has been approved for cryopreservation.

Quality Control

For each cryopreservation session, embryos from a control strain are also cryopreserved. This control sample is later thawed and tested for viability to provide quality control for the cryopreservation procedure.

Build: 2017.09.11 Non-profit supported by the Australian government  |  © Copyright 2017 Australian Phenomics Facility Last updated: 22/09/2017